Rapid Method for Isolation of DNA from Glass Slide Smears for PCR
نویسندگان
چکیده
منابع مشابه
A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymer...
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Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...
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Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملSlide PCR: DNA amplification from cell samples on microscopic glass slides.
The polymerase chain reaction (PCR) has been used to amplify sequences in polypropylene tubes from a wide range of template material. We have developed a method for amplifying DNA from cultured cells and smears directly on microscopic glass slides ('slide PCR'). Cells were either allowed to adhere as a monolayer or smeared onto silanised microscope slides (1), then fixed in methanol/acetic acid...
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Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...
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ژورنال
عنوان ژورنال: Acta Haematologica
سال: 1992
ISSN: 0001-5792,1421-9662
DOI: 10.1159/000204771